monoclonal antibodies anti pmtor (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc monoclonal antibodies anti pmtor
Monoclonal Antibodies Anti Pmtor, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 3459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pmtor/Phospho-mTOR+(Ser2448)+XP+Rabbit+mAb/pm41735269-95-50-55
Average 97 stars, based on 3459 article reviews

monoclonal antibodies anti pmtor - by Bioz Stars, 2026-09

97/100 stars

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Western Blot:

Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes
Article Snippet: Subsequently, cell lysis was carried out with RIPA lysis buffer (Beyotime, P0013B). .. The cell lysates were examined via SDS-PAGE, and immunoblotting was performed with the following antibodies: anti-pSYK (Cell Signaling Technology, CST, 2710S), anti-SYK (CST, 13198S), anti-pCD19 (CST, 3571S), anti-CD19 (CST, 90176S), anti-β-actin (Proteintech, 66009-1-Ig), anti-pNFκB pP65 (CST, 3033S), anti-NFκB P65 (CST, 4764S), anti-pSTAT1 (CST, 9167S), anti-STAT1 (CST, 14994S), anti-pSTAT3 (CST, 9145S), anti-STAT3 (CST, 9139S), anti-pSTAT5 (CST, 4322S), anti-STAT5 (CST, 94205S), anti-pPI3K pP85/pP55 (CST, 4228 L), anti-PI3K P85 (CST, 4292S), anti-pAKT (CST, 4060 L), anti-AKT (CST, 9272S), anti-pmTOR (CST, 5536S), anti-mTOR (CST, 2983S), anti-pS6 (CST, 4858 L), anti-S6 (CST, 2217S), anti-pFOXO1 (CST, 9461S), anti-FOXO1 (CST, 2880S), anti-CTCF (ABclonal, A19588), anti-SPIB (CST, 14337S), anti-ETS1 (ZenBio, R382074), anti-GABPA (HuaBio, HA500187 ), anti-FLI1 (HuaBio, HA500149 ), anti-PU.1 (ABclonal, A20461), anti-GAPDH (Proteintech, 60004-1-Ig), anti-PIK3C3 (ABclonal, A12295), anti-PTEN (CST, 9188S), anti-pSHIP-1 (CST, 3941S), SHIP-1 (ABclonal, A3571), anti-NOTCH2 (ABclonal, A24877), anti-pHSL (CST, 4126S), anti-HSL (CST, 4107S), anti-ATGL (CST, 2138S), anti-LC3 I/II (ABclonal, A5618), IκBα (ABclonal, A19714), pIκBα (SelleckChem, F2237), pIKKα/β (CST, 2697S), pP38 (SelleckChem, F0159), HSP90 (ABclonal, A5027), pJNK1/2 (ABclonal, AP0473), pERK1/2 (ABclonal, AP0472). ..

Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes.
Article Snippet: Subsequently, cell lysis was carried out with RIPA lysis buffer (Beyotime, P0013B). .. The cell lysates were examined via SDS-PAGE, and immunoblotting was performed with the following antibodies: anti-pSYK (Cell Signaling Technology, CST, 2710S), antiSYK (CST, 13198S), anti-pCD19 (CST, 3571S), anti-CD19 (CST, 90176S), anti-β-actin (Proteintech, 66009-1-Ig), anti-pNFκB pP65 (CST, 3033S), anti-NFκB P65 (CST, 4764S), anti-pSTAT1 (CST, 9167S), anti-STAT1 (CST, 14994S), anti-pSTAT3 (CST, 9145S), anti-STAT3 (CST, 9139S), anti-pSTAT5 (CST, 4322S), anti-STAT5 (CST, 94205S), anti-pPI3K pP85/pP55 (CST, 4228 L), anti-PI3K P85 (CST, 4292S), anti-pAKT (CST, 4060 L), anti-AKT (CST, 9272S), anti-pmTOR (CST, 5536S), anti-mTOR (CST, 2983S), anti-pS6 (CST, 4858 L), anti-S6 (CST, 2217S), anti-pFOXO1 (CST, 9461S), anti-FOXO1 (CST, 2880S), anti-CTCF (ABclonal, A19588), anti-SPIB (CST, 14337S), anti-ETS1 (ZenBio, R382074), anti-GABPA (HuaBio, HA500187), anti-FLI1 (HuaBio, HA500149), anti-PU.1 (ABclonal, A20461), anti-GAPDH (Proteintech, 60004-1-Ig), anti-PIK3C3 (ABclonal, A12295), antiPTEN (CST, 9188S), anti-pSHIP-1 (CST, 3941S), SHIP-1 (ABclonal, A3571), anti-NOTCH2 (ABclonal, A24877), anti-pHSL (CST, 4126S), anti-HSL (CST, 4107S), anti-ATGL (CST, 2138S), anti-LC3 I/II (ABclonal, A5618), IκBα (ABclonal, A19714), pIκBα (SelleckChem, F2237), pIKKα/β (CST, 2697S), pP38 (SelleckChem, F0159), HSP90 (ABclonal, A5027), pJNK1/2 (ABclonal, AP0473), pERK1/2 (ABclonal, AP0472). ..

Incubation:

Article Title: Metformin Ameliorates Cognitive Deficits and Neuroinflammation in a Mouse Model of Familial Hypercholesterolemia
Article Snippet: .. After incubation, the membranes were incubated overnight at 4 °C in a blocking solution containing one of the following antibodies: anti-mTOR (1:1000, Cell Signaling, Cat. no. #2983), anti-pmTOR (1:1000, Cell Signaling, Cat. no. #2971), anti-AMPK (1:1000, Abcam, Cat. no. #AB80039), anti-pAMPK (1:1000, Abcam, Cat. no. # AB133448 ), anti-PSD95 (1:1000, Cell Signaling, Cat. no. #36233), anti-Synaptophysin (1:1000, Sigma-Aldrich, Cat. no. #SAB4200544) or anti-β actin (1:1000, Sigma Aldrich, Cat. no. #A4700). .. The blot was then washed three times for 5 min with T TBS and incubated for 2 h in a solution containing peroxidase- conjugated anti- rabbit IgG (1:1000, Millipore, Cat. no. #AP132P) and peroxidase-conjugated anti-mouse IgG (1:1000, Millipore, Cat. no. #AP124P) or anti-rabbit IgG (1:1000, Millipore, Cat. no. #AP307P) and anti-mouse IgG (1:1000, Santa Cruz, Cat. no. #sc-516102).

Article Title: Metformin Ameliorates Cognitive Deficits and Neuroinflammation in a Mouse Model of Familial Hypercholesterolemia.
Article Snippet: .. After incubation, the membranes were incubated overnight at 4 °C in a blocking solution containing one of the following antibodies: anti-mTOR (1:1000, Cell Signaling, Cat. no. #2983), anti-pmTOR (1:1000, Cell Signaling, Cat. no. #2971), anti-AMPK (1:1000, Abcam, Cat. no. #AB80039), anti-pAMPK (1:1000, Abcam, Cat. no. #AB133448), anti-PSD95 (1:1000, Cell Signaling, Cat. no. ..

Article Title: β-glucan-loaded nano-niosomes ameliorate spatial and associative memory impairment in a D-galactose-induced aging rat model via modulation of microglial pyroptosis and autophagy.
Article Snippet: Equal protein amounts were resolved by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) (Bio-Rad Laboratories, USA) and transferred to 0.2 μm polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, USA). .. The membranes were blocked for 1 h at room temperature in 5 % bovine serum albumin (BSA; Cat. No: A- A7906; Sigma-Aldrich, USA) diluted in 0.1 % Tween-20 and then incubated overnight at 4 ◦C with the following primary antibodies: Anti-pPI3K (Cat. No: ab40755, Abcam), Anti-PI3K (Cat. No: 4292S, Cell Signaling), Anti-pAKT (Cat. No: ab38449, Abcam), Anti-AKT (Cat. No: ab8805, Abcam), Anti-pmTOR (Cat. No: 2971S, Cell Signaling), Anti-mTOR (Cat. No: ab134903, Abcam), Anti-β-actin (Cat. No: ab8227, Abcam). .. After washing, membranes were incubated with HRP-conjugated secondary antibody (1:10,000; Abcam, ab6721) for 1 h at room temperature.

Blocking Assay:

Staining:

Article Title: Senescent B cells regulate CD38 expression via FOXO1 in pneumonia resulting from PIK3CD (R437C) mutations
Article Snippet: .. Cells were washed with Perm/Wash Buffer (BD, 554723) to remove PFA and then stained with anti-pPI3K (4228S), anti-pFOXO1 (9461S), anti-pAKT (4060 L), anti-pS6 (4858S), anti-pmTOR (5536S), and anti-pCD19 for 30 min. Antibodies used were obtained from CST. ..

Review